Detail
Experiment ID:
EXP00322
Reference:
- Title: Toward the blood-borne miRNome of human diseases.
- Author: Keller A, Leidinger P, Bauer A, Elsharawy A, Haas J, Backes C, Wendschlag A, Giese N, Tjaden C, Ott K, Werner J, Hackert T, Ruprecht K, Huwer H, Huebers J, Jacobs G, Rosenstiel P, Dommisch H, Schaefer A, Müller-Quernheim J, Wullich B, Keck B, Graf N, Reichrath J, Vogel B, Nebel A, Jager SU, Staehler P, Amarantos I, Boisguerin V, Staehler C, Beier M, Scheffler M, Büchler MW, Wischhusen J, Haeusler SF, Dietl J, Hofmann S, Lenhof HP, Schreiber S, Katus HA, Rottbauer W, Meder B, Hoheisel JD, Franke A, Meese E
- Journal: Nature methods.2011 Sep 04;8(10):841-3.doi:10.1038/nmeth.1682.
- Abstract: "In a multicenter study, we determined the expression profiles of 863 microRNAs by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and validated this miRNome by quantitative real-time PCR. We detected consistently deregulated profiles for all tested diseases; pathway analysis confirmed disease association of the respective microRNAs. We observed significant correlations (P = 0.004) between the genomic location of disease-associated genetic variants and deregulated microRNAs."
- PMID: 21892151
Expression Profile:
- Description:The human Whole miRNOme project version 1
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE31568
- Platform: GPL9040
- Number of samples:453
- Overall design:A total of 454 samples was screened, containing patients with different cancer types (lung cancer, melanoma, prostate cancer, wilms tumors, tumor of stomach, pancreatic cancer, ovarian cancer), autoimmune diseases (multiple sclerosis), cardiovascular (acute myocardial infarction), and chronic inflammatory diseases (sarcoidosis, periodontitis, pancreatitis, chronic obstructive pulmonary diseases), as well as healthy control individuals. Please note that each miRNA has been measured in at least seven replicates and the median of the replica has been computed.
- Instrument:febit Homo Sapiens miRBase 13.0
Design and Sample:
- Cancer Type:pancreatic cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:blood
- Case Sample:blood from pancreatic cancer
- Control Sample:normal blood
- Num of Case:93
- Num of Control:70
- Quantification Software:limma
- Num of miRNAs:863
Identification:
- Num of Up:84
- Num of Down:135
Time Info:
- Create Time2016-03-14
- Update Time:2021-05-27
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|