Detail
Experiment ID:
EXP00441
Reference:
- Title: MicroRNAs associated with small bowel neuroendocrine tumours and their metastases.
- Author: Miller HC, Frampton AE, Malczewska A, Ottaviani S, Stronach EA, Flora R, Kaemmerer D, Schwach G, Pfragner R, Faiz O, Kos-Kudła B, Hanna GB, Stebbing J, Castellano L, Frilling A
- Journal: Endocrine-related cancer.2016 09 ;23(9):711-26.doi:10.1530/ERC-16-0044.
- Abstract: Novel molecular analytes are needed in small bowel neuroendocrine tumours (SBNETs) to better determine disease aggressiveness and predict treatment response. In this study, we aimed to profile the global miRNome of SBNETs, and identify microRNAs (miRNAs) involved in tumour progression for use as potential biomarkers. Two independent miRNA profiling experiments were performed (n=90), including primary SBNETs (n=28), adjacent normal small bowel (NSB; n=14), matched lymph node (LN) metastases (n=24), normal LNs (n=7), normal liver (n=2) and liver metastases (n=15). We then evaluated potentially targeted genes by performing integrated computational analyses. We discovered 39 miRNAs significantly deregulated in SBNETs compared with adjacent NSB. The most upregulated (miR-204-5p, miR-7-5p and miR-375) were confirmed by qRT-PCR. Two miRNAs (miR-1 and miR-143-3p) were significantly downregulated in LN and liver metastases compared with primary tumours. Furthermore, we identified upregulated gene targets for miR-1 and miR-143-3p in an existing SBNET dataset, which could contribute to disease progression, and show that these miRNAs directly regulate FOSB and NUAK2 oncogenes. Our study represents the largest global miRNA profiling of SBNETs using matched primary tumour and metastatic samples. We revealed novel miRNAs deregulated during SBNET disease progression, and important miRNA-mRNA interactions. These miRNAs have the potential to act as biomarkers for patient stratification and may also be able to guide treatment decisions. Further experiments to define molecular mechanisms and validate these miRNAs in larger tissue cohorts and in biofluids are now warranted.
- PMID: 27353039
Expression Profile:
- Description:Global miRNA expression in small bowel neuroendocrine tumors (SBNET) and their lymph node/liver metastases
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE70534
- Platform: GPL17537
- Number of samples:79
- Overall design:Dataset 1Matched samples from 15 SBNET patients (47 samples) were used to determine a miRNA profile for SBNET and identify potential markers of disease progression. Patient numbers: 1-15.Dataset 2A second dataset of miRNA expression data (43 samples) containing an increased number of liver metastases, as well as SBNET and lymph node metastases. Patient numbers: B9-B140, controls: C1, C2.
- Instrument:nCounter Human miRNA Expression Assay, V2
Design and Sample:
- Cancer Type:small intestinal neuroendocrine tumor
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:metastasis
- Case Sample:liver metastasis
- Control Sample:small bowel neuroendocrine tumors (SBNET)
- Num of Case:15
- Num of Control:28
- Quantification Software:Limma
- Num of miRNAs:800
Identification:
- Num of Up:47
- Num of Down:46
Time Info:
- Create Time2021-06-04
- Update Time:2021-06-07
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|