Detail
Experiment ID:
EXP00498
Reference:
- Title: Serum miR-626 and miR-5100 are Promising Prognosis Predictors for Oral Squamous Cell Carcinoma.
- Author: Shi J, Bao X, Liu Z, Zhang Z, Chen W, Xu Q
- Journal: Theranostics.2019;9(4):920-931.doi:10.7150/thno.30339.
- Abstract: Although serum microRNAs (miRNAs) are currently being considered as promising noninvasive biomarkers for cancers, their role in the prognosis of oral squamous cell carcinoma (OSCC) has not been elucidated. Here we aimed to identify serum miRNA biomarkers that could be used as prognosis predictors of OSCC. Methods: A cohort of 260 serum miRNA samples was assessed in a three-step approach that included a screening stage, a training stage, and a testing stage. The correlation between prognosis of OSCC and the miRNAs expression was comprehensively analyzed. Results: A two-miRNA signature involving miR-626 and miR-5100 has been developed. Patients defined to be high-risk group by the two-miRNA signature had significantly shortened median survival time compared with the low-risk group. In multivariate analysis, this two-miRNA signature was independently predictive of survival, and achieved a superior predictive value compared with that of traditional clinicopathologic factors such as pathology grade as well as tumor and node metastasis (TNM) stage. An integrated prognostic model combining the TNM stage and miRNA signature displayed the highest prognostic performance (AUC value: 0.787, specificity: 0.884, sensitivity: 0.573) compared to the TNM stage-alone (AUC value: 0.630, specificity: 0.526, sensitivity: 0.733) or miRNA signature-alone model (AUC value: 0.771, specificity: 0.768, sensitivity: 0.773). In addition, we found that OSCC tumor cells not only expressed a high level of these two miRNAs, but also secreted certain miRNAs into the extracellular environment, suggesting these miRNAs may originate from tumor cells. Conclusion: In our study, we established a two-miRNA signature that was strongly and independently associated with prognosis in OSCC, and may serve as a promising prognosis predictor.
- PMID: 30867806
Expression Profile:
- Description:Serum miRNA expression in 25 oral squamous cell cancer patients with different five years survival status and 15 healthy controls
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE113956
- Platform: GPL18058
- Number of samples:40
- Overall design:Total RNA was isolated using TRlzolTM LS Reagent (Invitrogen life technologies) from 40 individuals’ serum. RNA samples were analyzed by Exiqon A/S using miRCURYTM Hy3TM/Hy5TM power labeling kit and the miRCURYTM Array. Hybridization signals were scanned and analyzed by Genepix 4000B microarray scanner (Axon Instruments, Foster city, CA).
- Instrument:Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed Probe_ID version]
Design and Sample:
- Cancer Type:oral squamous cell carcinoma
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:blood
- Case Sample:cancer patient serum
- Control Sample:normal blood
- Num of Case:25
- Num of Control:15
- Quantification Software:Limma
- Num of miRNAs:1904
Identification:
- Num of Up:748
- Num of Down:664
Time Info:
- Create Time2021-06-04
- Update Time:2021-06-07
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
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