Detail
Experiment ID:
EXP00531
Reference:
- Title: Circulating miRNA panels for specific and early detection in bladder cancer.
- Author: Usuba W, Urabe F, Yamamoto Y, Matsuzaki J, Sasaki H, Ichikawa M, Takizawa S, Aoki Y, Niida S, Kato K, Egawa S, Chikaraishi T, Fujimoto H, Ochiya T
- Journal: Cancer science.2019 Jan ;110(1):408-419.doi:10.1111/cas.13856.
- Abstract: Bladder cancer is the 9th leading cause of cancer death worldwide. The major problem in bladder cancer is primarily the high recurrence rate after drug treatment and resection. Although conventional screening methods, such as cystoscopy, urinary cytology and ultrasound sonography, have become widely used in clinical settings, the diagnostic performance of these modalities is unsatisfactory due to low accuracy or high invasiveness. Because circulating micro RNA (miRNA) profiles have recently been reported as an attractive tool for liquid biopsy in cancer screening, here, we performed global miRNA profiling of 392 serum samples of bladder cancer patients with 100 non-cancer samples and 480 samples of other types of cancer as controls. We randomly classified the bladder cancer and control samples into 2 cohorts, a training set (N = 486) and a validation set (N = 486). By comparing both controls, we identified specific miRNA, such as miR-6087, for diagnosing bladder cancer in the training and validation sets. Furthermore, we found that a combination of 7 miRNA (7-miRNA panel: miR-6087, miR-6724-5p, miR-3960, miR-1343-5p, miR-1185-1-3p, miR-6831-5p and miR-4695-5p) could discriminate bladder cancer from non-cancer and other types of tumors with the highest accuracy (AUC: .97; sensitivity: 95%; specificity: 87%). The diagnostic accuracy was high, regardless of the stage and grade of bladder cancer. Our data demonstrated that the 7-miRNA panel could be a biomarker for the specific and early detection of bladder cancer.
- PMID: 30382619
Expression Profile:
- Description:Circulating miRNA panels for specific and early detection in bladder cancer
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE113486
- Platform: GPL21263
- Number of samples:972
- Overall design:Serum microRNA profiles of 972 samples, which consist of 392 bladder cancer, 100 non-cancer control, and 480 other types of cancer patients.
- Instrument:3D-Gene Human miRNA V21_1.0.0
Design and Sample:
- Cancer Type:colorectal cancer
- Cancer SubType:N/A
- Cell Line:N/A
- Experimental Design:blood
- Case Sample:blood from colorectal cancer
- Control Sample:normal blood
- Num of Case:40
- Num of Control:100
- Quantification Software:Limma
- Num of miRNAs:2565
Identification:
- Num of Up:471
- Num of Down:674
Time Info:
- Create Time2021-06-04
- Update Time:2021-06-07
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|