Detail
Experiment ID:
EXP00546
Reference:
- Title: Identification of Serum MicroRNAs as Novel Biomarkers in Esophageal Squamous Cell Carcinoma Using Feature Selection Algorithms.
- Author: Zheng D, Ding Y, Ma Q, Zhao L, Guo X, Shen Y, He Y, Wei W, Liu F
- Journal: Frontiers in oncology.2018;8:674.doi:10.3389/fonc.2018.00674.
- Abstract: Introduction: Circulating microRNAs (miRNAs) are promising molecular biomarkers for the early detection of esophageal squamous cell carcinoma (ESCC). We investigated the serum miRNA expression profiles from microarray-based technologies and evaluated the diagnostic value of serum miRNAs as potential biomarkers for ESCC by using feature selection algorithms. Methods: Serum miRNA expression profiles were obtained from 52 ESCC patients and 52 age- and sex-matched controls via performing a high-throughput microarray assay. Five representative feature selection algorithms including the false discovery rate procedure, family-wise error rate procedure, Lasso logistic regression, hybrid huberized support vector machine (SVM), and SVM using the squared-error loss with the elastic-net penalty were jointly carried out to select the significantly differentially expressed miRNAs based on the miRNA profiles. Results: Three miRNAs including miR-16-5p, miR-451a, and miR-574-5p were identified as the powerful biomarkers for the diagnosis of ESCC. The diagnostic accuracy of the combination of these three miRNAs was evaluated by using logistic regression and the SVM. The averages of the area under the receiver operating curve and classification accuracies based on different classifiers were more than 0.80 and 0.79, respectively. The cross-validation results suggested that the three-miRNA-based classifiers could clearly distinguish ESCC patients from healthy controls. Moreover, the classifying performance of the miRNA panel persisted in discriminating the healthy group from patients with ESCC stage I-II (AUC > 0.76) and patients with ESCC stage III-IV (AUC > 0.80). Conclusions: These results in this study have moved forward the identification of novel biomarkers for the diagnosis of ESCC.
- PMID: 30719423
Expression Profile:
- Description:Microarray expression profile of micro RNAs in serum for Esophageal Squamous Cell Carcinoma
- Organism:Homo sapiens
- Source:GEO
- Source ID:GSE112840
- Platform: GPL23365
- Number of samples:104
- Overall design:We have completed the Arraystar Human miRNA Array analysis of the 104 serum samples from preoperative ESCC patients (n=52) and healthy controls (n=52).
- Instrument:Agilent-046064 Human miRNA Microarray, Release 19.0, 8x60K (miRNA_ID version)
Design and Sample:
- Cancer Type:esophageal cancer
- Cancer SubType:esophageal squamous cell carcinomas
- Cell Line:N/A
- Experimental Design:blood
- Case Sample:blood from esophageal cancer
- Control Sample:normal blood
- Num of Case:52
- Num of Control:52
- Quantification Software:Limma
- Num of miRNAs:1897
Identification:
- Num of Up:325
- Num of Down:409
Time Info:
- Create Time2021-06-04
- Update Time:2021-06-07
Status:
| miRNA ID | Cancer Type | Design | logFC | AveExpr | T value | P value | adj Pvalue | Status | Plot |
|---|