Drug ID:Drug64
Drug Name:Methotrexate
CID:126941
DrugBank ID:DB00563
Modality:Small Molecule
Groups:approved
US Approved:YES
Other Approved:YES
Identifier: NCT00035074, , NCT02413047, , NCT01393405, , NCT00498589
Molecular Formula:C20H22N8O5
Molecular Weight:454.4 g/mol
Isomeric SMILES:CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
Synonyms:methotrexate; 59-05-2; Rheumatrex; Amethopterin; Methylaminopterin; Metatrexan; Hdmtx; Ledertrexate; Methylaminopterinum; MTX
Phase 0: 18
Phase 1: 204
Phase 2: 717
Phase 3: 465
Phase 4: 225
Description:An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of tetrahydrofolate dehydrogenase and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. [PubChem]

Molecular Structure

2D Molecular Structure

Knowledge Graph

dtIDCIDCompound NameGene IDGene NameSpeciesPubMed IDsAction
dt570 126941 Methotrexate 3417 IDH1 Homo sapiens (human) None
dt571 126941 Methotrexate 1014 CDH16 Homo sapiens (human) Inhibitor
dt572 126941 Methotrexate 10919 EHMT2 Homo sapiens (human) None
dt573 126941 Methotrexate 5423 POLB Homo sapiens (human) None
dt574 126941 Methotrexate 93777387 folA Escherichia coli K-12 16873019 None
dt575 126941 Methotrexate 1719 DHFR Homo sapiens (human) 12872145 DHFR protein polymorphism results in decreased susceptibility to folic acid antagonists
dt576 126941 Methotrexate 2940 GSTA3 Rattus norvegicus (Norway rat) 12872145 GSTA3 protein results in decreased susceptibility to folic acid antagonists
dt577 126941 Methotrexate 23435 TARDBP Homo sapiens (human) None
dt578 126941 Methotrexate 4780 NFE2L2 Homo sapiens (human) None

  • No data available

Phase Distribution
Phase Description
  • Phase 0: Exploratory trials to assess drug behavior in humans
  • Phase 1: Safety trials to determine safe dosage range
  • Phase 2: Efficacy trials to evaluate therapeutic effects
  • Phase 3: Large-scale trials to confirm efficacy and safety
  • Phase 4: Post-marketing surveillance for long-term safety and efficacy

Trial IDTitlePhaseStatusSponsorIndicationsInterventions
NCT00498589 Comparison of Methotrexate vs Placebo in Corticosteroid-dependent Ulcerative Colitis PHASE2 COMPLETED Centre Hospitalier Universitaire de Besancon Ulcerative Colitis DRUG: methotrexate|DRUG: placebo Details
NCT01393405 Methotrexate in Induction and Maintenance of Steroid Free Remission in Ulcerative Colitis PHASE2 COMPLETED University of North Carolina, Chapel Hill Ulcerative Colitis DRUG: Methotrexate Details
NCT04205734 Methotrexate Polyglutamate Measurements in Patients With Inflammatory Bowel Disease None SUSPENDED Boston Children's Hospital IBD DIAGNOSTIC_TEST: MTX Assessment Details
NCT04196920 Methotrexate-associated Anti-TNF Combination Therapy, the Neglected One ! None UNKNOWN University Hospital, Montpellier Chronic Inflammatory Bowel Disease None Details
NCT00035074 A Pharmacokinetic Study to Determine the Oral Bioavailability of Methotrexate in Patients With Inflammatory Bowel Disease None COMPLETED National Center for Research Resources (NCRR) Inflammatory Bowel Disease DRUG: Methotrexate Details
NL-OMON48064 Methotrexate to suppress immunogenicity to anti-tumor necrosis factor therapy in IBD patients with loss of response - Immunix Not Available None Academisch Medisch Centrum Inflammatory Bowel Disease (Crohn's Disease and U… Addition of methotrexaat to anti-therapy (inflixi… Details
NCT03860012 Evaluating the Efficacy of Weekly Folic Acid in Pediatric Inflammatory Bowel Disease Patients on Methotrexate PHASE4 Recruiting Le Bonheur Children's Hospital Inflammatory Bowel Diseases Drug: Folic Acid Details
NCT03204136 Tacrolimus Versus Methotrexate as Rescue Therapy for Refractory Inflammatory Bowel Disease: an Open-label, Retrospective Study Not Available Not recruiting Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, China Inflammatory Bowel Diseases;Inflammatory Bowel Di… None Details
ISRCTN23541842 The auxiliary diagnostic value of fissured tongue on axial and peripheral joint dysfunction in Ankylosing Spondylitis Not Available Not Recruiting Zhejiang University Of Chinese Medicine Ankylosing spondylitis Musculoskeletal Diseases … Participants are screened and undergo a clinical … Details
NCT03531593 Dose Effect Relationship of Methotrexate and Hepatic Fibrosis in Patients With Inflammatory Bowel Disease Not Available Not recruiting Children's Mercy Hospital Kansas City Inflammatory Bowel Diseases;Liver Fibroses;Methot… Diagnostic Test: Ultrasound Elastography Details
EUCTR2016-000522-18-FR Risk stratified randomized controlled trial in paediatric Crohn Disease: Methotrexate versus azathioprine or adalimumab for maintaining remission in patients at low or at high risk for aggressive disease course, respectively a treatment strategy - REDUCE-RISKincd-PIBD-TRIAL PHASE4 Not Available PIBDNet Crohn disease;Therapeutic area: Diseases [C] - Di… Product Name: humira Pharmaceutical Form: Solutio… Details
NCT02461784 The Effect of Methotrexate on Sperm Quality in Men With Inflammatory Bowel Disease None TERMINATED University of Wisconsin, Madison Inflammatory Bowel Disease (IBD) PROCEDURE: Semen & Data Collection Details
NCT05927064 Study Evaluating the Persistence, Efficacy and Tolerability of Methotrexate in Inflammatory Bowel Disease Patients None COMPLETED Central Hospital, Nancy, France Inflammatory Bowel Diseases|Crohn Disease|Ulcerat… None Details
NCT02413047 Evaluate if Response to Infliximab or Adalimumab May be Regained With an Immunomodulator None TERMINATED Indiana University Inflammatory Bowel Disease|Ulcerative Colitis|Cro… DRUG: Azathioprine|DRUG: 6 mercaptopurine|DRUG: M… Details
NCT01716039 Pharmacokinetics of Adalimumab With Methotrexate for Treatment of Patients With Ulcerative Colitis (UC) PHASE2 TERMINATED Alimentiv Inc. Ulcerative Colitis DRUG: MTX 12.5|DRUG: MTX 25|DRUG: Adalimumab Details

Disease IDDisease NameDefinitionCategoryRelated DrugsMechanism
No data available

Strategy IDTherapeutic StrategySynonymsRelated DrugsMechanism
S02 Immunosuppression Classic immunosuppressive drugs azathioprine; 6-mercaptopurine; methotrexate; ciclosporin-A; tacrolimus Ciclosporin-A and tacrolimus bind to specific intracellular… Details

Therapeutic role of methotrexate in pediatric Crohn's disease

PMID: 29338679
Year: 2018
Relationship Type: Treatment Score: 7.3

The main role of therapy in Crohn's disease (CD) is to achieve long-term clinical remission, and to allow for normal growth and development of childr…

The Influence of Methotrexate Treatment on Male Fertility and Pregnancy Outcome…

PMID: 28267049
Year: 2017
Relationship Type: Treatment Score: 7.3

BACKGROUND: Inflammatory bowel disease incidence peaks during the reproductive years. Methotrexate (MTX) is frequently used for inflammatory bowel di…

Methotrexate Polyglutamate Monitoring in Patients With Crohn's Disease

PMID: 27274011
Year: 2017
Relationship Type: Treatment Score: 7.3

Methotrexate is an efficacious immunosuppressant for induction and maintenance of remission in Crohn's disease. The goal of this pilot study was to d…

Methotrexate for Inflammatory Bowel Diseases - New Developments

PMID: 26981630
Year: 2016
Relationship Type: Treatment Score: 7.3

Methotrexate (MTX) is an established therapy for patients with steroid-dependent Crohn's disease (CD). MTX is also frequently used in combination wit…

[A Case of Crohn's Disease Showing Favorable Response to Induction and Maintena…

PMID: 26493510
Year: 2015
Relationship Type: Treatment Score: 7.3

Thanks to the introduction of immumomodulators and biologics, therapeutic approaches in Crohn's disease have changed significantly during the past de…

Use of Methotrexate in the Treatment of Inflammatory Bowel Diseases

PMID: 26457382
Year: 2016
Relationship Type: Treatment Score: 7.3

Low-dose methotrexate (MTX) therapy is a well-recognized therapy for many inflammatory conditions such as rheumatoid arthritis, psoriatic arthritis, …

Methotrexate for maintaining remission in paediatric Crohn's patients with prio…

PMID: 25656249
Year: 2015
Relationship Type: Treatment Score: 7.3

BACKGROUND AND AIMS: Methotrexate [MTX] is an immunomodulating drug that can be used to maintain remission in patients with Crohn's disease [CD], but…

Methotrexate: an old new drug in autoimmune disease

PMID: 25245537
Year: 2014
Relationship Type: Treatment Score: 7.3

Methotrexate (MTX) is currently considered, among disease-modifying anti-rheumatic drugs (DMARDs), the 'anchor-drug' in the treatment of rheumatoid a…

[The efficacy and safety of methotrexate in refractory Crohn's disease]

PMID: 24767205
Year: 2014
Relationship Type: Treatment Score: 7.3

OBJECTIVE: To assess the efficacy and safety of methotrexate(MTX) on refractory Crohn's disease(CD). METHODS: A total of 35 consecutive refractory CD…

Pharmacokinetic profiles for oral and subcutaneous methotrexate in patients wit…

PMID: 23190184
Year: 2013
Relationship Type: Adverse Effect Score: 7.1

BACKGROUND: Methotrexate (MTX) is administered subcutaneously to Crohn's Disease (CD) patients. There are very few studies evaluating the use of oral…

Evaluation of liver fibrosis by transient elastography in methotrexate treated …

PMID: 21719043
Year: 2011
Relationship Type: Treatment Score: 7.1

BACKGROUND AND AIMS: Methotrexate (MTX) safety is questioned by the risk of inducing liver fibrosis (LF). As transient elastography (FibroScan(R)) is…

Short-course ondansetron for the prevention of methotrexate-induced nausea in c…

PMID: 21681112
Year: 2011
Relationship Type: Treatment Score: 7.1

OBJECTIVES: Methotrexate (MTX) is an effective treatment for Crohn disease (CD); however, its application may be limited by the occurrence of nausea.…

Mucosal healing with methotrexate in Crohn's disease: a prospective comparative…

PMID: 21235604
Year: 2011
Relationship Type: Treatment Score: 7.1

BACKGROUND: Mucosal healing has become a new therapeutic goal in Crohn's disease and can be achieved with azathioprine (AZA) or biologics. Methotrexa…

Use of methotrexate in patients with inflammatory bowel diseases

PMID: 21044450
Year: 2010
Relationship Type: Treatment Score: 7.1

Methotrexate (MTX) is one of the immunosuppressants commonly used in inflammatory bowel diseases. There is very good evidence for its use in patients…

Methotrexate for maintenance of remission in chronic active Crohn's disease: lo…

PMID: 19924799
Year: 2010
Relationship Type: Treatment Score: 7.1

BACKGROUND: According to current guidelines methotrexate (MTX) should be considered as a second-line immunomodulator in patients with chronic active …

Methotrexate and Tumor Necrosis Factor Inhibitors Independently Decrease Neutra…

PMID: 39340091
Year: 2024
Relationship Type: Association Score: 6.5

Objective: SARS-CoV-2 remains the third most common cause of death in North America. We studied the effects of methotrexate and tumor necrosis factor…

Systematic review: Methotrexate-A poorly understood and underused medication in…

PMID: 39076140
Year: 2024
Relationship Type: Treatment Score: 6.5

BACKGROUND: Methotrexate, an immunosuppressant used for the treatment of inflammatory bowel disease (IBD) for over 30 years, remains underused compar…

Vedolizumab, Adalimumab, and Methotrexate Combination Therapy in Crohn's Diseas…

PMID: 37743037
Year: 2024
Relationship Type: Treatment Score: 6.5

BACKGROUND & AIMS: Although biologics have revolutionized the treatment of Crohn's disease (CD), an efficacy ceiling has been reached. Combining biol…

Effectiveness and tolerability of methotrexate monotherapy in Crohn's disease p…

PMID: 37655055
Year: 2023
Relationship Type: Treatment Score: 6.5

BACKGROUND: Methotrexate monotherapy is recommended as a maintenance therapy for Crohn's disease (CD). However, long-term follow-up data are scarce. …

The Role of Low-Dose Oral Methotrexate in Increasing Anti-TNF Drug Levels and R…

PMID: 37445417
Year: 2023
Relationship Type: Treatment Score: 6.5

Concomitant immunomodulation is utilised in combination with anti-TNF therapy for IBD primarily to increase drug levels and prevent anti-drug antibod…

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