Drug ID:Drug64
Drug Name:Methotrexate
CID:126941
DrugBank ID:DB00563
Modality:Small Molecule
Groups:approved
US Approved:YES
Other Approved:YES
Identifier: NCT00035074, , NCT02413047, , NCT01393405, , NCT00498589
Molecular Formula:C20H22N8O5
Molecular Weight:454.4 g/mol
Isomeric SMILES:CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
Synonyms:methotrexate; 59-05-2; Rheumatrex; Amethopterin; Methylaminopterin; Metatrexan; Hdmtx; Ledertrexate; Methylaminopterinum; MTX
Phase 0: 18
Phase 1: 204
Phase 2: 717
Phase 3: 465
Phase 4: 225
Description:An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of tetrahydrofolate dehydrogenase and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. [PubChem]

Molecular Structure

2D Molecular Structure

Knowledge Graph

dtIDCIDCompound NameGene IDGene NameSpeciesPubMed IDsAction
dt570 126941 Methotrexate 3417 IDH1 Homo sapiens (human) None
dt571 126941 Methotrexate 1014 CDH16 Homo sapiens (human) Inhibitor
dt572 126941 Methotrexate 10919 EHMT2 Homo sapiens (human) None
dt573 126941 Methotrexate 5423 POLB Homo sapiens (human) None
dt574 126941 Methotrexate 93777387 folA Escherichia coli K-12 16873019 None
dt575 126941 Methotrexate 1719 DHFR Homo sapiens (human) 12872145 DHFR protein polymorphism results in decreased susceptibility to folic acid antagonists
dt576 126941 Methotrexate 2940 GSTA3 Rattus norvegicus (Norway rat) 12872145 GSTA3 protein results in decreased susceptibility to folic acid antagonists
dt577 126941 Methotrexate 23435 TARDBP Homo sapiens (human) None
dt578 126941 Methotrexate 4780 NFE2L2 Homo sapiens (human) None

  • No data available

Phase Distribution
Phase Description
  • Phase 0: Exploratory trials to assess drug behavior in humans
  • Phase 1: Safety trials to determine safe dosage range
  • Phase 2: Efficacy trials to evaluate therapeutic effects
  • Phase 3: Large-scale trials to confirm efficacy and safety
  • Phase 4: Post-marketing surveillance for long-term safety and efficacy

Trial IDTitlePhaseStatusSponsorIndicationsInterventions
NCT07138898 Immunosuppressant Management in Rheumatology Patients Undergoing Elective Total Shoulder Arthroplasty PHASE2 NOT_YET_RECRUITING NYU Langone Health Rheumatic Disease DRUG: Methotrexate|DRUG: Sulfasalazine|DRUG: Hydr… Details
NCT05072782 Management of Moderate POstoperative Recurrence in Crohn's Disease: a randoMizEd contROLled Trial of Therapeutic Escalation, the POMEROL Trial. (POMEROL) PHASE4 UNKNOWN Groupe d'Etude Therapeutique des Affections Inflammatoires Digestives Crohn Disease DRUG: Infliximab CT-P13|DRUG: Immunosuppressors (… Details
NCT05040464 Comparison of Azathioprine to Methotrexate in Combination Therapy With Adalimumab in Crohn's Disease: an Open-label Randomized Controlled Trial PHASE3 RECRUITING Centre Hospitalier Universitaire, Amiens Crohn Disease|Azathioprine|Methotrexate DRUG: AZA capsules|DRUG: MTX|BIOLOGICAL: blood sa… Details
NCT04205734 Methotrexate Polyglutamate Measurements in Patients With Inflammatory Bowel Disease NULL SUSPENDED Boston Children's Hospital IBD DIAGNOSTIC_TEST: MTX Assessment Details
NCT04196920 Methotrexate-associated Anti-TNF Combination Therapy, the Neglected One ! NULL UNKNOWN University Hospital, Montpellier Chronic Inflammatory Bowel Disease None Details
NCT03531593 Dose Effect Relationship of MTX and IBD NULL COMPLETED Children's Mercy Hospital Kansas City Inflammatory Bowel Diseases|Liver Fibroses|Methot… DIAGNOSTIC_TEST: Ultrasound Elastography Details
NCT03204136 Tacrolimus Versus Methotrexate as Rescue Therapy for Refractory Inflammatory Bowel Disease NULL UNKNOWN Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, China Inflammatory Bowel Diseases None Details
NCT02852694 Reduce Risk for Crohn's Disease Patients PHASE4 COMPLETED PIBD-Net Crohn's Disease DRUG: Methotrexate|DRUG: Adalimumab|DRUG: Azathio… Details
NCT02764762 Triple Combination Therapy in High Risk Crohn's Disease (CD) PHASE4 COMPLETED Takeda Crohn Disease DRUG: Vedolizumab|DRUG: Adalimumab|DRUG: Methotre… Details
NCT02461784 The Effect of Methotrexate on Sperm Quality in Men With Inflammatory Bowel Disease NULL TERMINATED University of Wisconsin, Madison Inflammatory Bowel Disease (IBD) PROCEDURE: Semen & Data Collection Details
NCT02413047 Evaluate if Response to Infliximab or Adalimumab May be Regained With an Immunomodulator NA TERMINATED Indiana University Inflammatory Bowel Disease|Ulcerative Colitis|Cro… DRUG: Azathioprine|DRUG: 6 mercaptopurine|DRUG: M… Details
NCT02269358 Salvage Therapy With High/Low Methotrexate for Loss of Response to Infliximab Dose Escalation PHASE4 COMPLETED Prof. Arie Levine Crohn's Disease DRUG: METHOTREXATE Details
NCT02177071 A proSpective Randomized Controlled Trial comParing infliximAb-antimetabolites Combination Therapy to Anti-metabolites monotheRapy and Infliximab monothErapy in Crohn's Disease Patients in Sustained Steroid-free Remission on Combination Therapy PHASE4 COMPLETED Groupe d'Etude Therapeutique des Affections Inflammatoires Digestives Crohn's Disease DRUG: INFLIXIMAB|DRUG: AZATHIOPRINE|DRUG: MERCAPT… Details
NCT01802593 Comparison of 2 Immunomodulator Withdrawal Schemes for Infliximab Monotherapy in Active Pediatric Crohn's Disease After Thiopurine Failure PHASE4 TERMINATED Prof. Arie Levine Crohn's Disease DRUG: AZATHIOPRINE or METHOTREXATE|DRUG: AZATHIOP… Details
NCT01716039 Pharmacokinetics of Adalimumab With Methotrexate for Treatment of Patients With Ulcerative Colitis (UC) PHASE2 TERMINATED Alimentiv Inc. Ulcerative Colitis DRUG: MTX 12.5|DRUG: MTX 25|DRUG: Adalimumab Details
NCT01393405 Methotrexate in Induction and Maintenance of Steroid Free Remission in Ulcerative Colitis PHASE2 COMPLETED University of North Carolina, Chapel Hill Ulcerative Colitis DRUG: Methotrexate Details
NCT00546546 Early Immunosuppressants in Crohn's Disease PHASE4 COMPLETED Groupe d'Etude Therapeutique des Affections Inflammatoires Digestives Crohn's Disease DRUG: early immunosuppressants (azathioprine, met… Details
NCT00498589 Comparison of Methotrexate vs Placebo in Corticosteroid-dependent Ulcerative Colitis PHASE2 COMPLETED Centre Hospitalier Universitaire de Besancon Ulcerative Colitis DRUG: methotrexate|DRUG: placebo Details
NCT00132899 COMMIT (Combination Of Maintenance Methotrexate-Infliximab Trial) PHASE3 COMPLETED University of Western Ontario, Canada Crohn's Disease DRUG: Methotrexate|DRUG: Placebo Details
NCT00035074 A Pharmacokinetic Study to Determine the Oral Bioavailability of Methotrexate in Patients With Inflammatory Bowel Disease NULL COMPLETED National Center for Research Resources (NCRR) Inflammatory Bowel Disease DRUG: Methotrexate Details

Disease IDDisease NameDefinitionCategoryRelated DrugsMechanism
No data available

Strategy IDTherapeutic StrategySynonymsRelated DrugsMechanism
S02 Immunosuppression Classic immunosuppressive drugs azathioprine; 6-mercaptopurine; methotrexate; ciclosporin-A; tacrolimus Ciclosporin-A and tacrolimus bind to specific intracellular… Details

Therapeutic role of methotrexate in pediatric Crohn's disease

PMID: 29338679
Year: 2018
Relationship Type: Treatment Score: 7.3

The main role of therapy in Crohn's disease (CD) is to achieve long-term clinical remission, and to allow for normal growth and development of childr…

The Influence of Methotrexate Treatment on Male Fertility and Pregnancy Outcome…

PMID: 28267049
Year: 2017
Relationship Type: Treatment Score: 7.3

BACKGROUND: Inflammatory bowel disease incidence peaks during the reproductive years. Methotrexate (MTX) is frequently used for inflammatory bowel di…

Methotrexate Polyglutamate Monitoring in Patients With Crohn's Disease

PMID: 27274011
Year: 2017
Relationship Type: Treatment Score: 7.3

Methotrexate is an efficacious immunosuppressant for induction and maintenance of remission in Crohn's disease. The goal of this pilot study was to d…

Methotrexate for Inflammatory Bowel Diseases - New Developments

PMID: 26981630
Year: 2016
Relationship Type: Treatment Score: 7.3

Methotrexate (MTX) is an established therapy for patients with steroid-dependent Crohn's disease (CD). MTX is also frequently used in combination wit…

[A Case of Crohn's Disease Showing Favorable Response to Induction and Maintena…

PMID: 26493510
Year: 2015
Relationship Type: Treatment Score: 7.3

Thanks to the introduction of immumomodulators and biologics, therapeutic approaches in Crohn's disease have changed significantly during the past de…

Use of Methotrexate in the Treatment of Inflammatory Bowel Diseases

PMID: 26457382
Year: 2016
Relationship Type: Treatment Score: 7.3

Low-dose methotrexate (MTX) therapy is a well-recognized therapy for many inflammatory conditions such as rheumatoid arthritis, psoriatic arthritis, …

Methotrexate for maintaining remission in paediatric Crohn's patients with prio…

PMID: 25656249
Year: 2015
Relationship Type: Treatment Score: 7.3

BACKGROUND AND AIMS: Methotrexate [MTX] is an immunomodulating drug that can be used to maintain remission in patients with Crohn's disease [CD], but…

Methotrexate: an old new drug in autoimmune disease

PMID: 25245537
Year: 2014
Relationship Type: Treatment Score: 7.3

Methotrexate (MTX) is currently considered, among disease-modifying anti-rheumatic drugs (DMARDs), the 'anchor-drug' in the treatment of rheumatoid a…

[The efficacy and safety of methotrexate in refractory Crohn's disease]

PMID: 24767205
Year: 2014
Relationship Type: Treatment Score: 7.3

OBJECTIVE: To assess the efficacy and safety of methotrexate(MTX) on refractory Crohn's disease(CD). METHODS: A total of 35 consecutive refractory CD…

Pharmacokinetic profiles for oral and subcutaneous methotrexate in patients wit…

PMID: 23190184
Year: 2013
Relationship Type: Adverse Effect Score: 7.1

BACKGROUND: Methotrexate (MTX) is administered subcutaneously to Crohn's Disease (CD) patients. There are very few studies evaluating the use of oral…

Evaluation of liver fibrosis by transient elastography in methotrexate treated …

PMID: 21719043
Year: 2011
Relationship Type: Treatment Score: 7.1

BACKGROUND AND AIMS: Methotrexate (MTX) safety is questioned by the risk of inducing liver fibrosis (LF). As transient elastography (FibroScan(R)) is…

Short-course ondansetron for the prevention of methotrexate-induced nausea in c…

PMID: 21681112
Year: 2011
Relationship Type: Treatment Score: 7.1

OBJECTIVES: Methotrexate (MTX) is an effective treatment for Crohn disease (CD); however, its application may be limited by the occurrence of nausea.…

Mucosal healing with methotrexate in Crohn's disease: a prospective comparative…

PMID: 21235604
Year: 2011
Relationship Type: Treatment Score: 7.1

BACKGROUND: Mucosal healing has become a new therapeutic goal in Crohn's disease and can be achieved with azathioprine (AZA) or biologics. Methotrexa…

Use of methotrexate in patients with inflammatory bowel diseases

PMID: 21044450
Year: 2010
Relationship Type: Treatment Score: 7.1

Methotrexate (MTX) is one of the immunosuppressants commonly used in inflammatory bowel diseases. There is very good evidence for its use in patients…

Methotrexate for maintenance of remission in chronic active Crohn's disease: lo…

PMID: 19924799
Year: 2010
Relationship Type: Treatment Score: 7.1

BACKGROUND: According to current guidelines methotrexate (MTX) should be considered as a second-line immunomodulator in patients with chronic active …

Methotrexate and Tumor Necrosis Factor Inhibitors Independently Decrease Neutra…

PMID: 39340091
Year: 2024
Relationship Type: Association Score: 6.5

Objective: SARS-CoV-2 remains the third most common cause of death in North America. We studied the effects of methotrexate and tumor necrosis factor…

Systematic review: Methotrexate-A poorly understood and underused medication in…

PMID: 39076140
Year: 2024
Relationship Type: Treatment Score: 6.5

BACKGROUND: Methotrexate, an immunosuppressant used for the treatment of inflammatory bowel disease (IBD) for over 30 years, remains underused compar…

Vedolizumab, Adalimumab, and Methotrexate Combination Therapy in Crohn's Diseas…

PMID: 37743037
Year: 2024
Relationship Type: Treatment Score: 6.5

BACKGROUND & AIMS: Although biologics have revolutionized the treatment of Crohn's disease (CD), an efficacy ceiling has been reached. Combining biol…

Effectiveness and tolerability of methotrexate monotherapy in Crohn's disease p…

PMID: 37655055
Year: 2023
Relationship Type: Treatment Score: 6.5

BACKGROUND: Methotrexate monotherapy is recommended as a maintenance therapy for Crohn's disease (CD). However, long-term follow-up data are scarce. …

The Role of Low-Dose Oral Methotrexate in Increasing Anti-TNF Drug Levels and R…

PMID: 37445417
Year: 2023
Relationship Type: Treatment Score: 6.5

Concomitant immunomodulation is utilised in combination with anti-TNF therapy for IBD primarily to increase drug levels and prevent anti-drug antibod…

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