Drug ID:Drug64
Drug Name:Methotrexate
CID:126941
DrugBank ID:DB00563
Modality:Small Molecule
Groups:approved
US Approved:YES
Other Approved:YES
Identifier: NCT00035074, , NCT02413047, , NCT01393405, , NCT00498589
Molecular Formula:C20H22N8O5
Molecular Weight:454.4 g/mol
Isomeric SMILES:CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
Synonyms:methotrexate; 59-05-2; Rheumatrex; Amethopterin; Methylaminopterin; Metatrexan; Hdmtx; Ledertrexate; Methylaminopterinum; MTX
Phase 0: 18
Phase 1: 204
Phase 2: 717
Phase 3: 465
Phase 4: 225
Description:An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of tetrahydrofolate dehydrogenase and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. [PubChem]

Molecular Structure

2D Molecular Structure

Knowledge Graph

dtIDCIDCompound NameGene IDGene NameSpeciesPubMed IDsAction
dt570 126941 Methotrexate 3417 IDH1 Homo sapiens (human) None
dt571 126941 Methotrexate 1014 CDH16 Homo sapiens (human) Inhibitor
dt572 126941 Methotrexate 10919 EHMT2 Homo sapiens (human) None
dt573 126941 Methotrexate 5423 POLB Homo sapiens (human) None
dt574 126941 Methotrexate 93777387 folA Escherichia coli K-12 16873019 None
dt575 126941 Methotrexate 1719 DHFR Homo sapiens (human) 12872145 DHFR protein polymorphism results in decreased susceptibility to folic acid antagonists
dt576 126941 Methotrexate 2940 GSTA3 Rattus norvegicus (Norway rat) 12872145 GSTA3 protein results in decreased susceptibility to folic acid antagonists
dt577 126941 Methotrexate 23435 TARDBP Homo sapiens (human) None
dt578 126941 Methotrexate 4780 NFE2L2 Homo sapiens (human) None

  • No data available

Phase Distribution
Phase Description
  • Phase 0: Exploratory trials to assess drug behavior in humans
  • Phase 1: Safety trials to determine safe dosage range
  • Phase 2: Efficacy trials to evaluate therapeutic effects
  • Phase 3: Large-scale trials to confirm efficacy and safety
  • Phase 4: Post-marketing surveillance for long-term safety and efficacy

Trial IDTitlePhaseStatusSponsorIndicationsInterventions
NCT00498589 Comparison of Methotrexate vs Placebo in Corticosteroid-dependent Ulcerative Colitis PHASE2 COMPLETED Centre Hospitalier Universitaire de Besancon Ulcerative Colitis DRUG: methotrexate|DRUG: placebo Details
NCT01393405 Methotrexate in Induction and Maintenance of Steroid Free Remission in Ulcerative Colitis PHASE2 COMPLETED University of North Carolina, Chapel Hill Ulcerative Colitis DRUG: Methotrexate Details
NCT04205734 Methotrexate Polyglutamate Measurements in Patients With Inflammatory Bowel Disease None SUSPENDED Boston Children's Hospital IBD DIAGNOSTIC_TEST: MTX Assessment Details
NCT04196920 Methotrexate-associated Anti-TNF Combination Therapy, the Neglected One ! None UNKNOWN University Hospital, Montpellier Chronic Inflammatory Bowel Disease None Details
NCT00035074 A Pharmacokinetic Study to Determine the Oral Bioavailability of Methotrexate in Patients With Inflammatory Bowel Disease None COMPLETED National Center for Research Resources (NCRR) Inflammatory Bowel Disease DRUG: Methotrexate Details
NL-OMON48064 Methotrexate to suppress immunogenicity to anti-tumor necrosis factor therapy in IBD patients with loss of response - Immunix Not Available None Academisch Medisch Centrum Inflammatory Bowel Disease (Crohn's Disease and U… Addition of methotrexaat to anti-therapy (inflixi… Details
NCT03860012 Evaluating the Efficacy of Weekly Folic Acid in Pediatric Inflammatory Bowel Disease Patients on Methotrexate PHASE4 Recruiting Le Bonheur Children's Hospital Inflammatory Bowel Diseases Drug: Folic Acid Details
NCT03204136 Tacrolimus Versus Methotrexate as Rescue Therapy for Refractory Inflammatory Bowel Disease: an Open-label, Retrospective Study Not Available Not recruiting Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, China Inflammatory Bowel Diseases;Inflammatory Bowel Di… None Details
ISRCTN23541842 The auxiliary diagnostic value of fissured tongue on axial and peripheral joint dysfunction in Ankylosing Spondylitis Not Available Not Recruiting Zhejiang University Of Chinese Medicine Ankylosing spondylitis Musculoskeletal Diseases … Participants are screened and undergo a clinical … Details
NCT03531593 Dose Effect Relationship of Methotrexate and Hepatic Fibrosis in Patients With Inflammatory Bowel Disease Not Available Not recruiting Children's Mercy Hospital Kansas City Inflammatory Bowel Diseases;Liver Fibroses;Methot… Diagnostic Test: Ultrasound Elastography Details
EUCTR2016-000522-18-FR Risk stratified randomized controlled trial in paediatric Crohn Disease: Methotrexate versus azathioprine or adalimumab for maintaining remission in patients at low or at high risk for aggressive disease course, respectively a treatment strategy - REDUCE-RISKincd-PIBD-TRIAL PHASE4 Not Available PIBDNet Crohn disease;Therapeutic area: Diseases [C] - Di… Product Name: humira Pharmaceutical Form: Solutio… Details
NCT02461784 The Effect of Methotrexate on Sperm Quality in Men With Inflammatory Bowel Disease None TERMINATED University of Wisconsin, Madison Inflammatory Bowel Disease (IBD) PROCEDURE: Semen & Data Collection Details
NCT05927064 Study Evaluating the Persistence, Efficacy and Tolerability of Methotrexate in Inflammatory Bowel Disease Patients None COMPLETED Central Hospital, Nancy, France Inflammatory Bowel Diseases|Crohn Disease|Ulcerat… None Details
NCT02413047 Evaluate if Response to Infliximab or Adalimumab May be Regained With an Immunomodulator None TERMINATED Indiana University Inflammatory Bowel Disease|Ulcerative Colitis|Cro… DRUG: Azathioprine|DRUG: 6 mercaptopurine|DRUG: M… Details
NCT01716039 Pharmacokinetics of Adalimumab With Methotrexate for Treatment of Patients With Ulcerative Colitis (UC) PHASE2 TERMINATED Alimentiv Inc. Ulcerative Colitis DRUG: MTX 12.5|DRUG: MTX 25|DRUG: Adalimumab Details

Disease IDDisease NameDefinitionCategoryRelated DrugsMechanism
No data available

Strategy IDTherapeutic StrategySynonymsRelated DrugsMechanism
S02 Immunosuppression Classic immunosuppressive drugs azathioprine; 6-mercaptopurine; methotrexate; ciclosporin-A; tacrolimus Ciclosporin-A and tacrolimus bind to specific intracellular… Details

Neuroradiological worsening of brain abscess after adalimumab and methotrexate …

PMID: 30611629
Year: 2019
Relationship Type: Treatment Score: 6.5

The incidence of brain abscess is higher among immunosuppressed patients. We report a case of a brain abscess in the temporal lobe in a patient under…

Higher Mucosal Healing with Tumor Necrosis Factor Inhibitors in Combination wit…

PMID: 30560332
Year: 2019
Relationship Type: Treatment Score: 6.5

BACKGROUND: The differential impact of anti-tumor necrosis factor (anti-TNF) therapy with methotrexate versus thiopurine co-therapy on endoscopic rem…

Update on the Use of Thiopurines and Methotrexate in Inflammatory Bowel Disease

PMID: 30267281
Year: 2018
Relationship Type: Treatment Score: 6.3

PURPOSE OF REVIEW: The increased use of biologic agents over the past two decades has led to a reappraisal of the role of the immunomodulators (thiop…

Methotrexate and lung disease in rheumatoid arthritis

PMID: 27711025
Year: 2017
Relationship Type: Treatment Score: 6.3

Rheumatoid arthritis (RA) is a common chronic inflammatory disease affecting many tissues and resulting in substantial morbidity and increased mortal…

Review of the bioanalytical methods for the determination of methotrexate and i…

PMID: 27623319
Year: 2017
Relationship Type: Treatment Score: 6.3

Methotrexate is an old drug that has found use in several therapeutic areas, such as cancer to treat various malignancies, rheumatoid arthtritis and …

Inadvertent yellow fever vaccination of a patient with Crohn's disease treated …

PMID: 27571912
Year: 2016
Relationship Type: Treatment Score: 6.3

We present a case of a 56-year-old woman with Crohn's disease, treated with methotrexate and infliximab, who inadvertently received yellow fever vacc…

Safety profile of methotrexate in inflammatory bowel disease

PMID: 27462711
Year: 2016
Relationship Type: Treatment Score: 6.3

INTRODUCTION: Methotrexate, which was initially developed in 1948 for the treatment of leukemia, is known to be an immunomodulatory and anti-inflamma…

A case of enteropathic arthritis successfully treated with methotrexate

PMID: 27320938
Year: 2016
Relationship Type: Association Score: 6.3

We report a 36-year-old male patient who suffered from polyarthritis after the remission of ulcerative colitis. He was diagnosed with enteropathic ar…

Molluscum contagiosum of the eyelid: case report in a man receiving methotrexat…

PMID: 27136625
Year: 2016
Relationship Type: Treatment Score: 6.3

BACKGROUND: Molluscum contagiosum is a benign viral infection of the skin. Lesions typically present as dome-shaped, flesh-colored, umbilicated papul…

European experience with methotrexate treatment in Crohn's disease: a multicent…

PMID: 26894634
Year: 2016
Relationship Type: Treatment Score: 6.3

Comment in Gastroenterology. 2015 May;148(5):877-80. BACKGROUND & AIMS: The medical management of ulcerative colitis (UC) has improved through …

Methotrexate for the Management of Crohn's Disease in Children

PMID: 26511908
Year: 2016
Relationship Type: Treatment Score: 6.3

OBJECTIVE: To review the literature evaluating methotrexate as a treatment option for Crohn's disease (CD) in pediatric patients. DATA SOURCES: A sea…

Risk of liver injury among methotrexate users: A meta-analysis of randomised co…

PMID: 26088004
Year: 2015
Relationship Type: Treatment Score: 6.3

OBJECTIVE: Methotrexate is an effective treatment for a variety of inflammatory diseases. Robust evidence on the risk of serious liver injury is lack…

Methotrexate use and risk of lung disease in psoriasis, psoriatic arthritis, an…

PMID: 25770113
Year: 2015
Relationship Type: Adverse Effect Score: 6.3

OBJECTIVE: To evaluate the relative risk of pulmonary disease among patients with psoriasis, psoriatic arthritis, and inflammatory bowel disease trea…

[Reccomendations of the Spanish Working Group on Crohn's Disease and Ulcerative…

PMID: 25454602
Year: 2015
Relationship Type: Treatment Score: 6.3

Methotrexate is an immunosuppressant that may be useful in several clinical scenarios in inflammatory bowel disease. In this article, we review the a…

Methotrexate for maintenance of remission in Crohn's disease

PMID: 25157445
Year: 2014
Relationship Type: Treatment Score: 6.3

Update of Cochrane Database Syst Rev. 2007;(4):CD006618. BACKGROUND: Ulcerative colitis (UC) is a chronic inflammatory bowel disease. Corticost…

Methotrexate: underused and ignored?

PMID: 23295701
Year: 2012
Relationship Type: Treatment Score: 6.1

For more than a decade, methotrexate has been known to be an effective therapeutic agent in the treatment of steroid-dependent active Crohn's disease…

Efficacy and tolerability of methotrexate therapy for refractory Crohn's diseas…

PMID: 22112005
Year: 2012
Relationship Type: Treatment Score: 6.1

BACKGROUND: Randomised controlled trials demonstrate that methotrexate is effective in inducing remission and preventing relapse of Crohn's disease (…

The use of methotrexate for treatment of inflammatory bowel disease in clinical…

PMID: 22051323
Year: 2012
Relationship Type: Treatment Score: 6.1

BACKGROUND: Methotrexate is considered a treatment for Crohn's disease, whilst few data in ulcerative colitis are available. AIM: To evaluate frequen…

Incidence of liver toxicity in inflammatory bowel disease patients treated with…

PMID: 21751301
Year: 2012
Relationship Type: Adverse Effect Score: 6.1

Update in Cochrane Database Syst Rev. 2015;8:CD007560. BACKGROUND: Methotrexate, a folate antagonist, is an immunosuppressant drug that is effe…

Methotrexate: a drug of the future in ulcerative colitis?

PMID: 21466494
Year: 2011
Relationship Type: Treatment Score: 6.1

Ulcerative colitis (UC) is a chronic inflammatory bowel disease that is thought to affect 1780000 patients in USA and Europe. Its incidence is increa…

Showing 61-80 of 88 articles