Drug ID:Drug64
Drug Name:Methotrexate
CID:126941
DrugBank ID:DB00563
Modality:Small Molecule
Groups:approved
US Approved:YES
Other Approved:YES
Identifier: NCT00035074, , NCT02413047, , NCT01393405, , NCT00498589
Molecular Formula:C20H22N8O5
Molecular Weight:454.4 g/mol
Isomeric SMILES:CN(CC1=CN=C2C(=N1)C(=NC(=N2)N)N)C3=CC=C(C=C3)C(=O)N[C@@H](CCC(=O)O)C(=O)O
Synonyms:methotrexate; 59-05-2; Rheumatrex; Amethopterin; Methylaminopterin; Metatrexan; Hdmtx; Ledertrexate; Methylaminopterinum; MTX
Phase 0: 18
Phase 1: 204
Phase 2: 717
Phase 3: 465
Phase 4: 225
Description:An antineoplastic antimetabolite with immunosuppressant properties. It is an inhibitor of tetrahydrofolate dehydrogenase and prevents the formation of tetrahydrofolate, necessary for synthesis of thymidylate, an essential component of DNA. [PubChem]

Molecular Structure

2D Molecular Structure

Knowledge Graph

dtIDCIDCompound NameGene IDGene NameSpeciesPubMed IDsAction
dt570 126941 Methotrexate 3417 IDH1 Homo sapiens (human) None
dt571 126941 Methotrexate 1014 CDH16 Homo sapiens (human) Inhibitor
dt572 126941 Methotrexate 10919 EHMT2 Homo sapiens (human) None
dt573 126941 Methotrexate 5423 POLB Homo sapiens (human) None
dt574 126941 Methotrexate 93777387 folA Escherichia coli K-12 16873019 None
dt575 126941 Methotrexate 1719 DHFR Homo sapiens (human) 12872145 DHFR protein polymorphism results in decreased susceptibility to folic acid antagonists
dt576 126941 Methotrexate 2940 GSTA3 Rattus norvegicus (Norway rat) 12872145 GSTA3 protein results in decreased susceptibility to folic acid antagonists
dt577 126941 Methotrexate 23435 TARDBP Homo sapiens (human) None
dt578 126941 Methotrexate 4780 NFE2L2 Homo sapiens (human) None

  • No data available

Phase Distribution
Phase Description
  • Phase 0: Exploratory trials to assess drug behavior in humans
  • Phase 1: Safety trials to determine safe dosage range
  • Phase 2: Efficacy trials to evaluate therapeutic effects
  • Phase 3: Large-scale trials to confirm efficacy and safety
  • Phase 4: Post-marketing surveillance for long-term safety and efficacy

Trial IDTitlePhaseStatusSponsorIndicationsInterventions
NCT07138898 Immunosuppressant Management in Rheumatology Patients Undergoing Elective Total Shoulder Arthroplasty PHASE2 NOT_YET_RECRUITING NYU Langone Health Rheumatic Disease DRUG: Methotrexate|DRUG: Sulfasalazine|DRUG: Hydr… Details
NCT05072782 Management of Moderate POstoperative Recurrence in Crohn's Disease: a randoMizEd contROLled Trial of Therapeutic Escalation, the POMEROL Trial. (POMEROL) PHASE4 UNKNOWN Groupe d'Etude Therapeutique des Affections Inflammatoires Digestives Crohn Disease DRUG: Infliximab CT-P13|DRUG: Immunosuppressors (… Details
NCT05040464 Comparison of Azathioprine to Methotrexate in Combination Therapy With Adalimumab in Crohn's Disease: an Open-label Randomized Controlled Trial PHASE3 RECRUITING Centre Hospitalier Universitaire, Amiens Crohn Disease|Azathioprine|Methotrexate DRUG: AZA capsules|DRUG: MTX|BIOLOGICAL: blood sa… Details
NCT04205734 Methotrexate Polyglutamate Measurements in Patients With Inflammatory Bowel Disease NULL SUSPENDED Boston Children's Hospital IBD DIAGNOSTIC_TEST: MTX Assessment Details
NCT04196920 Methotrexate-associated Anti-TNF Combination Therapy, the Neglected One ! NULL UNKNOWN University Hospital, Montpellier Chronic Inflammatory Bowel Disease None Details
NCT03531593 Dose Effect Relationship of MTX and IBD NULL COMPLETED Children's Mercy Hospital Kansas City Inflammatory Bowel Diseases|Liver Fibroses|Methot… DIAGNOSTIC_TEST: Ultrasound Elastography Details
NCT03204136 Tacrolimus Versus Methotrexate as Rescue Therapy for Refractory Inflammatory Bowel Disease NULL UNKNOWN Key Laboratory of Gastroenterology and Hepatology, Ministry of Health, China Inflammatory Bowel Diseases None Details
NCT02852694 Reduce Risk for Crohn's Disease Patients PHASE4 COMPLETED PIBD-Net Crohn's Disease DRUG: Methotrexate|DRUG: Adalimumab|DRUG: Azathio… Details
NCT02764762 Triple Combination Therapy in High Risk Crohn's Disease (CD) PHASE4 COMPLETED Takeda Crohn Disease DRUG: Vedolizumab|DRUG: Adalimumab|DRUG: Methotre… Details
NCT02461784 The Effect of Methotrexate on Sperm Quality in Men With Inflammatory Bowel Disease NULL TERMINATED University of Wisconsin, Madison Inflammatory Bowel Disease (IBD) PROCEDURE: Semen & Data Collection Details
NCT02413047 Evaluate if Response to Infliximab or Adalimumab May be Regained With an Immunomodulator NA TERMINATED Indiana University Inflammatory Bowel Disease|Ulcerative Colitis|Cro… DRUG: Azathioprine|DRUG: 6 mercaptopurine|DRUG: M… Details
NCT02269358 Salvage Therapy With High/Low Methotrexate for Loss of Response to Infliximab Dose Escalation PHASE4 COMPLETED Prof. Arie Levine Crohn's Disease DRUG: METHOTREXATE Details
NCT02177071 A proSpective Randomized Controlled Trial comParing infliximAb-antimetabolites Combination Therapy to Anti-metabolites monotheRapy and Infliximab monothErapy in Crohn's Disease Patients in Sustained Steroid-free Remission on Combination Therapy PHASE4 COMPLETED Groupe d'Etude Therapeutique des Affections Inflammatoires Digestives Crohn's Disease DRUG: INFLIXIMAB|DRUG: AZATHIOPRINE|DRUG: MERCAPT… Details
NCT01802593 Comparison of 2 Immunomodulator Withdrawal Schemes for Infliximab Monotherapy in Active Pediatric Crohn's Disease After Thiopurine Failure PHASE4 TERMINATED Prof. Arie Levine Crohn's Disease DRUG: AZATHIOPRINE or METHOTREXATE|DRUG: AZATHIOP… Details
NCT01716039 Pharmacokinetics of Adalimumab With Methotrexate for Treatment of Patients With Ulcerative Colitis (UC) PHASE2 TERMINATED Alimentiv Inc. Ulcerative Colitis DRUG: MTX 12.5|DRUG: MTX 25|DRUG: Adalimumab Details
NCT01393405 Methotrexate in Induction and Maintenance of Steroid Free Remission in Ulcerative Colitis PHASE2 COMPLETED University of North Carolina, Chapel Hill Ulcerative Colitis DRUG: Methotrexate Details
NCT00546546 Early Immunosuppressants in Crohn's Disease PHASE4 COMPLETED Groupe d'Etude Therapeutique des Affections Inflammatoires Digestives Crohn's Disease DRUG: early immunosuppressants (azathioprine, met… Details
NCT00498589 Comparison of Methotrexate vs Placebo in Corticosteroid-dependent Ulcerative Colitis PHASE2 COMPLETED Centre Hospitalier Universitaire de Besancon Ulcerative Colitis DRUG: methotrexate|DRUG: placebo Details
NCT00132899 COMMIT (Combination Of Maintenance Methotrexate-Infliximab Trial) PHASE3 COMPLETED University of Western Ontario, Canada Crohn's Disease DRUG: Methotrexate|DRUG: Placebo Details
NCT00035074 A Pharmacokinetic Study to Determine the Oral Bioavailability of Methotrexate in Patients With Inflammatory Bowel Disease NULL COMPLETED National Center for Research Resources (NCRR) Inflammatory Bowel Disease DRUG: Methotrexate Details

Disease IDDisease NameDefinitionCategoryRelated DrugsMechanism
No data available

Strategy IDTherapeutic StrategySynonymsRelated DrugsMechanism
S02 Immunosuppression Classic immunosuppressive drugs azathioprine; 6-mercaptopurine; methotrexate; ciclosporin-A; tacrolimus Ciclosporin-A and tacrolimus bind to specific intracellular… Details

The safety and tolerability of methotrexate for treating patients with Crohn's …

PMID: 21224737
Year: 2011
Relationship Type: Treatment Score: 6.1

GOALS: To determine the safety and tolerance of methotrexate for treating patients with Crohn's disease in clinical practice. BACKGROUND: Methotrexat…

Methotrexate-induced pneumonitis in a patient with Crohn's disease

PMID: 21122509
Year: 2010
Relationship Type: Treatment Score: 6.1

Pulmonary toxicity is a well recognised but infrequent adverse event of treatment with methotrexate. The vast majority of cases have occurred in pati…

Methotrexate in paediatric ulcerative colitis: a retrospective survey at a sing…

PMID: 20937047
Year: 2010
Relationship Type: Adverse Effect Score: 6.1

BACKGROUND: Patients with ulcerative colitis often receive thiopurines as immunomodulators (IMs) to maintain remission and avoid corticosteroids. If …

Combined approach with infliximab, surgery, and methotrexate in severe fistuliz…

PMID: 20623697
Year: 2011
Relationship Type: Treatment Score: 6.1

BACKGROUND: Infliximab is the only medical therapy that has been proven to be effective in fistulizing Crohn's disease (CD), but the recurrence rate …

Clinical experience of methotrexate in Crohn's disease: response, safety and mo…

PMID: 20354043
Year: 2010
Relationship Type: Treatment Score: 6.1

BACKGROUND: Methotrexate is an immunosuppressant used in the treatment of patients with Crohn's disease who are intolerant or refractory to azathiopr…

Efficacy of methotrexate in ulcerative colitis: failure or promise

PMID: 20186931
Year: 2010
Relationship Type: Treatment Score: 6.1

BACKGROUND: Low-dose methotrexate is a widely used and efficacious therapy in chronic inflammatory disorders such as psoriasis and rheumatoid arthrit…

Methotrexate for induction of remission in ulcerative colitis

PMID: 17943916
Year: 2007
Relationship Type: Treatment Score: 6.1

Update in Cochrane Database Syst Rev. 2018 Aug 11;8:CD005984. BACKGROUND: Prevention of relapse is a major issue in the management of Crohn's d…

[The use of methotrexate in an inflammatory bowel diseases based on the review …

PMID: 27487545
Year: 2016
Relationship Type: Treatment Score: 5.3

M ethotrexate (MTX) as an immunomodulatory drug has numerous applications in autoimmune diseases. Autoimmune patomechanism is one of the factors resp…

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