In vitro & In vivo Models

In vitro & in vivo models of NAFLD are manually extracted from research articles.

68 records.
Model ID Category Class Type Model Description Source Reference (PMIDs)
M041 In vitro Tissue culture Liver-on-a-chip Microfluidic device HepG2 cells were cultured into a microfluidically perfused device under free fatty acid (FFA) supplementation, namely palmitic and oleic acid, for 24h and 48h. Human 27438262
M042 In vitro Tissue culture Liver-on-a-chip Human liver Coculturing human hepatocytes, umbilical vein endothelial cells (HUVECs), and Kupffer cells (KCs) into spheroids Human 31746151
M043 In vitro Tissue culture Liver-on-a-chip Multicellular liver microtissues Composed of four major types of liver cells (hepatocytes, endothelial cells, Kupffer cells, and stellate cells) to implement a human hepatic fibrosis model driven by NAFLD: i) lipid accumulation in hepatocytes (steatosis), ii) neovascularization by endothelial cells, iii) inflammation by activated Kupffer cells (steatohepatitis), and iv) extracellular matrix deposition by activated stellate cells (fibrosis). Human 33690979
M044 In vitro Tissue culture Liver-on-a-chip NASH-on-a-chip Four main types of human primary liver cells (hepatocytes [HCs], Kupffer cells, liver sinusoidal endothelial cells, and hepatic stellate cells [HSCs]) were cocultured under microfluidic dynamics. Human 33553970
M045 In vitro Tissue culture Liver-on-a-chip Microphysiological system Human in vitro co-culture NASH model using primary human hepatocytes, Kupffer cells and hepatic stellate cells, which are cultured together as microtissues in a perfused three-dimensional microphysiological system (MPS). Human 31909357
M046 In vitro Tissue culture Body-on-a-chip Gut-liver on a chip A conventional in vitro model for hepatic steatosis uses a liver cell culture, treated with fatty acids and measures accumulation of lipids within the cells. Human 29981260
M047 In vitro Tissue culture Body-on-a-chip Human-on-a-chip Human-on-a-chip model composed of human hepatocytes and adipose tissue chambers capable of modeling the metabolic factors that contribute to liver disease development and progression, and evaluation of the therapeutic metformin. Human 34162924
M048 In vitro Tissue culture Bio-print Human liver tissue Scaffold-free 3D bio-printing technology to construct a small portion of liver tissue Human 28955746
M049 In vitro Tissue culture Bio-print Primary liver tissue Bioprinted human liver tissue mimetics comprised of patient-derived hepatocytes and non-parenchymal cells in a defined architecture. Human 27387377
M050 In vivo Animal Dietary mouse High-fat diet feeding A stable isogenic cross between C57BL/6J (B6) and 129S1/SvImJ (S129) mice were fed a high fat diet with ad libitum consumption of glucose and fructose in physiologically relevant concentrations and compared to mice fed a chow diet and also to both parent strains. Mouse 27261415
M051 In vivo Animal Dietary mouse Fast-food diet feeding A diet on the basis of the composition of "fast food" (high saturated fats, cholesterol, and fructose). Mice (n = 8 in each group) were assigned to diets as follows: 1) standard chow (SC), i.e., 13% energy as fat [1% saturated fatty acids (SFA)], 2) high fat (HF), i.e., 60% energy as fat (1% SFA), and 3) fast food (FF), i.e., 40% energy as fat (12% SFA, 2% cholesterol). Mouse 21836057
M052 In vivo Animal Dietary mouse Methionine-choline-deficient (MCD) diet feeding Male C57BL/6 mice were fed an MCD diet for 30 weeks. A recovery model was also established wherein a normal control diet was provided for 2 weeks after a period of 8, 16, or 30 weeks. Mouse 24294355
M053 In vivo Animal Dietary mouse Choline-deficient, ethionine-supplemented (CDE) dietary Separately administering choline-deficient chow and ethionine in the drinking water (CD+E diet) Mouse 26496771
M054 In vivo Animal Dietary mouse Choline-deficient L-amino acid-defined (CDAA) diet Choline-deficient diet in which proteins are replaced with an equimolar mixture of L-amino acids Mouse 16931074
M055 In vivo Animal Dietary mouse STAM Neonatal male mice exposed to low-dose streptozotocin (STZ) developed liver steatosis with diabetes 1 week after feeding high-fat diet (HFD) Mouse 23430399
M056 In vivo Animal Genetic mouse ob/ob Mice (Leptin deficiency) leptin-deficient obese (Lep(ob)/Lep(ob)) mouse Mouse 11914033
M057 In vivo Animal Genetic mouse db/db Mice (Leptin receptor deficiency) db/db mice and the corresponding lean and nondiabetic db/m mice were fed a diet deficient in methionine and choline (MCD diet) or control diet for 4 wk. Leptin-deficient obese and diabetic ob/ob mice fed similar diets were used for comparison. Mouse 15256362
M058 In vivo Animal Genetic mouse Mc4r-Deficient Mice Melanocortin 4 receptor-deficient mice (MC4R-KO) Mouse 21906580
M059 In vivo Animal Genetic mouse Srebp1c-Overexpressing Mice Liver-specific transgenic (Tg) mice overexpressing the active form of sterol response element binding protein-1c (SREBP-1c) fed a high-fat and fructose diet (HFFD) Mouse 24129399
M060 In vivo Animal Genetic mouse FATZO Mice Male FATZO mice at the age of 8 weeks were fed with high fat Western diet (D12079B) supplemented with 5% fructose in the drinking water (WDF) for the duration of 20 weeks. Mouse 30885145