Project IDOEP005128
Project NameSingle cell RNA-seq for immune cells in TME
Experiment TypeTranscriptomic
OEX027419
Project IDOEP005129
Project NameSingle cell RNA-seq for immune cells in TME-2
Experiment TypeTranscriptomic
OEX027420
Project IDOEP004112
Project Name测试项目
Experiment TypeGenomic
ProtocolNuclei from formaldehyde crosslinked (1%; 10min at room temperature) embryos were isolated and chromatin was fragmented using sonicator (Branson). Lysates were cleared and protein-DNA complexes were isolated using target specific antibodies and Dynabeads. DNA fragments were purified and libraries were prepared by NEBnext DNA Library Prep Kit (NEB, E7370L). Adapter ligated DNA was purified using AMPure XP beads (Beckman Coulter, A63881) and sequenced by illumina HiSeq 2500. NEBnext DNA Library Prep Kit
OEX023087
Project IDOEP001025
Project Nameprecise medicine
Experiment TypeGenomic
OEX014352
Project IDOEP004015
Project Name16S rRNA gene diversity during DOES incubation under different HHP
Experiment TypeMetagenomic
OEX025544
Project IDOEP004015
Project Name16S rRNA gene diversity during DOES incubation under different HHP
Experiment TypeMetagenomic
OEX022552
Project IDOEP004042
Project NameMetagenomics during DOES incubation under different HHP
Experiment TypeMetagenomic
ProtocolThe extracted microbial DNA was processed to construct metagenome shotgun sequencing libraries following the standard Illumina TruSeq DNA Sample Preparation Guide. Each library was sequenced by Illumina NovaSeq 6000 platform (Illumina, USA) with PE150 strategy at Shanghai Personal Biotechnology (Shanghai, China).
OEX022859
Project IDOEP004045
Project NameMetatranscriptomics during DOES incubation under different HHP
Experiment TypeMetatranscriptomic
ProtocolTo ensure DNA removal, the RNA extracts are treated with TURBO DNase (Invitrogen, Waltham, MA, USA) as directed by the manufacturer.
OEX022922
Project IDOEP004102
Project NameHeterologous expression and activity assay of clade I and II N2O reductase genes using WP3 strain at 0.1, 20 and 40 MPa
Experiment TypeTranscriptomic
OEX023021
Project IDOEP005097
Project NameDifferentiation of islet organoids (S5D3)
Experiment TypeTranscriptomic
ProtocolAll experiments were performed according to Trizol nucleic acid extraction, GEO seq transcriptome amplification, and TruePrep® DNA Library Prep Kit V2 for Illumina® instruction manual. The experiments were carried out to extract RNA using Trizol method from organoids. Smart II and KAPA amplification were used, BGI P100 was sequenced, and sheared to approximately 300-500 bp using Q-sep instrument.
OEX027384